Eye tracking is an important tool in usability testing of a screen-based user interface. Though eye tracking has been used in usability testing for quite a while, challenges remain. For example, how to accurately calibrate gaze point? How to interpret a scan pattern? In this workshop, we will introduce the basics of the human oculomotor system, the role of eye tracking in cognition, eye tracking recording techniques, and data analysis methods. Upon the completion of this workshop, students will have a basic understanding of physiological and psychological mechanisms underlying eye tracking, data collection techniques, and data analysis methods. The workshop will consist of five modules as described below: Human oculomotor system, visual attention and eye tracking, eye tracking data collection, data analysis, and a case study.